Your sample may absorb light, scatter light, or both. Uv vis spectroscopy spectroscopy molecular physics. The interaction of molecules with ultraviolet and visible light may results in absorption of photons. Modern laser spectroscopy allows the study reactions on timescales of ms, s, ns, ps and even fs. Timeresolved spectroscopy the application of time resolved spectroscopy has become an important tool for the study of shortlived reactive intermediates. Absorption spectra were collected using a cary iii uvvis spectrophotometer. The visible spectrum 390780 nm represents only a small portion of the. Uv vis nir spectroscopy analysis of nanoparticles 4. Region wavelength nm far ultraviolet 10200 near ultraviolet 200380 visible 380780 near infrared 7803000. The example below covers the range of nano to microseconds. Pdf components, principle and applications of uv vis. Uv visible spectroscopy and attenuated total reflectance fourier. The deeper the color, the more light will be absorbed.
Scribd is the worlds largest social reading and publishing site. Basic uvvis theory, concepts and applications page 2 of 28 for convenience of reference, definitions of the various spectral regions have been set by the joint committee on nomenclature in applied spectroscopy. Gamma radiation, with the smallest wavelength 1 pm, to low frequency radiation, with the largest wavelength well. Absorption spectroscopy involves measuring, via a spectrometer, the intensity of light after it passes through a colored solution. The promoted electrons are electrons of the highest molecular orbitals homo. A simple uvvis absorption spectrum displaying the peak absorption wavelength. Incident light from the source is focused on the sample. The course of the reaction is monitored via the formation of triiodide i3.
Uv vis spectroscopy free download as powerpoint presentation. Variable thickness thinlayer cell for electrochemistry. Although the uv spectrum extends below 100 nm high energy. Scattering and absorption the relative percentage of scatter or absorption from the measured extinction spectrum depends on the size, shape, composition and aggregation state of your sample. Visible spectroscopy is the study of the interaction of radiation from the visible part. This results in electronic transition, involving valance electrons, from ground state to higher electronic states called excited states. Determination of wine color by uvvis spectroscopy, johan. A variable thickness thin layer spectroelectrochemical cell for in situ uv vis absorption, luminescence and surfaceenhanced raman spectroscopy measurement is described. Absorptions of uvvis photons by molecule results in electronic excitation of molecule with chromophore. Basic uvvis theory, concepts and applications uni salzburg. Visible spectroscopy revised 112114 5 a beers law plot is a calibration curve of absorption plotted as a function of concentration. The uvvis spectrometer we will use consists of a white light source, a sample compartment and a detector. All the spectral data in photochemcad 3 are stored as text files. Pdf uvvis spectrophotometry fundamentals and applications.
An absorption spectrum must be acquired first to determine the wavelength of maximum absorbance, max, for the compound being studied. For ultraviolet and visible wavelengths, one should expect from this discussion that the absorption spectrum of a molecule i. In uv spectroscopy, the sample is irradiated with the broad spectrum of the uv. How can i obtain the uvvis spectrum using gaussian 09. Pdf in this applicationoriented booklet the fundamentals of uvvis. Comparative dataset of experimental and computational attributes of. The electronic transition involves promotion of electron from a electronic ground state to higher energy state. Components, principle and applications of uv vis spectophotometer. Database of absorption and fluorescence spectra of 300 common. In a drs spectrum, the ratio of the light scattered from an in. The primary environmental purpose in determining the ultravioletvisible uvvis absorption spectrum of a chemical compound is to have. It is constructed of chemically resistant polytetrafluoroethylene ptfe materials.